larvicidal activities of indigenous bacillus thuringiensis isolates and nematode symbiotic bacterial toxins against the mosquito vector, culex pipiens (diptera: culicidae)

نویسندگان

ashraf m ahmed department of zoology, college of science, king saud university, riyadh, saudi arabia department of zoology, college of science, minia university, el-minia, egypt

hamdy i hussein department of plant protection, college of food and agricultural sciences, king saud university, riyadh, saudi arabia

talat a el-kersh department of clinical laboratory sciences, college of applied medical sciences, king saud university, riyadh, saudi arabia

yazeed a al-sheikh department of clinical laboratory sciences, college of applied medical sciences, king saud university, riyadh, saudi arabia

چکیده

background: the incidence of mosquito-borne diseases and the resistance of mosquitoes to conventional pesticides have recently caused a panic to the authorities in the endemic countries. this study was conducted to identify native larvicidal biopesticides against culex pipiens for utilization in the battle against mosquito-borne diseases. methods: larvicidal activities of new indigenous bacillus thuringiensis isolates and crude toxin complexes (tcs) of two nematode bacterial-symbionts, photorhabdus luminescens akhurstii (hrm1) and ph. luminescens akhurstii (hs1) that tested against cx. pipiens . b. thuringiensis isolates were recovered from different environmental samples in saudi arabia, and the entomopathogenic nematodes, heterorhabditis indica (hrm1) and he. sp (hs1) were iso­lated from egypt. larvicidal activities (lc 50 and lc 95 ) of the potentially active b. thuringiensis strains or tcs were then evaluated at 24 and 48h post-treatment. results: three b. thuringiensis isolates were almost as active as the reference b. thuringiensis israelensis (bti-h14), and seven isolates were 1.6–5.4 times more toxic than bti-h14. on the other hand, the tcs of the bacterial sym­bionts, hrm1 and hs1, showed promising larvicidal activities. hs1 showed lc 50 of 2.54 folds that of hrm1 at 24h post-treatment. moreover, histopathological examinations of the hs1-treated larvae showed deformations in midgut epithelial cells at 24h post-treatment. conclusion: synergistic activity and molecular characterization of these potentially active biocontrol agents are currently being investigated. these results may lead to the identification of eco-friend mosquito larvicidal product(s) that could contribute to the battle against mosquito-borne diseases.

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عنوان ژورنال:
journal of arthropod-borne diseases

جلد ۲۰۱۵، شماره ۱۰، صفحات ۰-۰

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